Hotspot

trnK-UUU-rps16

LSC · 4214-5056 · 843 bp

Back to Solidago

Pi 0.00119

MarkerSeek score 59.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 843
variable_sites: 1
pi: 0.00119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCCTAGCCGCACTTAAAAGC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	964-968 bp	1.0	75.7
trnK-UUU-rps16_p2	CCGCACTTAAAAGCCGAGTA	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.3	58.3	958-962 bp	1.0	75.6
trnK-UUU-rps16_p3	TCGCGGTCTTCCAAACTTTA	TTCCTTGAAAAAGGCGCTCA	45.0	45.0	57.5	58.3	1030-1034 bp	1.0	73.6
trnK-UUU-rps16_p4	CGCGGTCTTCCAAACTTTAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.4	58.3	1029-1033 bp	1.0	73.1
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	TCCTTGAAAAAGGCGCTCAG	50.0	50.0	58.3	58.8	963-967 bp	1.0	72.1