Hotspot

ycf1

SSC · 125358-131021 · 5664 bp

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Pi 0.0262

MarkerSeek score 67.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5664
variable_sites: 1807
pi: 0.0262
unique_haplotype_count: 176
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTCAGCTTCCATTCCCCAA	CCCCCTTTTTGTCGACAGAA	50.0	50.0	58.1	58.0	560-647 bp	0.928934	88.3
ycf1_p2	TCAGCTTCCATTCCCCAAAC	CCCCCTTTTTGTCGACAGAA	50.0	50.0	58.1	58.0	558-645 bp	0.928934	88.3
ycf1_p3	AGCTTCCATTCCCCAAACTG	CCCCCTTTTTGTCGACAGAA	50.0	50.0	58.1	58.0	556-643 bp	0.923858	88.2
ycf1_p4	CAGCTTCCATTCCCCAAACT	CCCCCTTTTTGTCGACAGAA	50.0	50.0	58.1	58.0	557-644 bp	0.923858	88.2
ycf1_p5	TCCCGTCTCTCAACCAATCT	CCCCCTTTTTGTCGACAGAA	50.0	50.0	58.1	58.0	452-506 bp	0.482234	68.1