Hotspot

rpl32-trnL-UAG

SSC · 114695-115615 · 921 bp

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Pi 0.0328

MarkerSeek score 70.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 921
variable_sites: 376
pi: 0.0328
unique_haplotype_count: 140
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CAGTGAATCGAACGGGGATT	AAGTAGCAAAGCCTTGGGTC	50.0	50.0	58.0	58.1	1877-2238 bp	0.873096	86.0
rpl32-trnL-UAG_p2	CAGTGAATCGAACGGGGATT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1878-2239 bp	0.873096	86.0
rpl32-trnL-UAG_p3	CAGTGAATCGAACGGGGATT	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	58.0	58.1	1879-2240 bp	0.873096	86.0
rpl32-trnL-UAG_p4	CAGTGAATCGAACGGGGATT	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	1390-1751 bp	0.873096	85.7
rpl32-trnL-UAG_p5	CAGTGAATCGAACGGGGATT	TCGAACCGAGATGCATTAGC	50.0	50.0	58.0	58.1	1416-1777 bp	0.873096	85.6