Hotspot

rps12-psbB

LSC · 71793-74383 · 2591 bp

Back to Solanum

Pi 0.0257

MarkerSeek score 69.90

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 2591
variable_sites: 583
pi: 0.0257
unique_haplotype_count: 155
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	TGACATTTCTGATTGGCTGTCT	CGATACCAAGGCAAACCCAT	40.9	50.0	58.0	58.2	2639-2751 bp	1.0	73.9
rps12-psbB_p2	GTGACATTTCTGATTGGCTGTC	CGATACCAAGGCAAACCCAT	45.5	50.0	57.9	58.2	2640-2752 bp	1.0	73.4
rps12-psbB_p3	GTGACATTTCTGATTGGCTGT	CGATACCAAGGCAAACCCAT	42.9	50.0	56.8	58.2	2640-2752 bp	1.0	72.5
rps12-psbB_p4	TGACATTTCTGATTGGCTGTCT	ACACGATACCAAGGCAAACC	40.9	50.0	58.0	58.5	2642-2754 bp	1.0	72.0
rps12-psbB_p5	GTGACATTTCTGATTGGCTGTC	ACACGATACCAAGGCAAACC	45.5	50.0	57.9	58.5	2643-2755 bp	1.0	71.5