Hotspot

petA-psbJ

LSC · 64936-66026 · 1091 bp

Back to Solanum

Pi 0.0341

MarkerSeek score 70.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1091
variable_sites: 384
pi: 0.0341
unique_haplotype_count: 138
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCAGAGCTATCGCCCCAATA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2075-2356 bp	0.959391	90.2
petA-psbJ_p2	CGGATTTGGTCAGGGAGATG	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1580-1858 bp	0.903553	87.8
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1579-1857 bp	0.903553	87.8
petA-psbJ_p4	TCAGAGCTATCGCCCCAATA	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1976-2254 bp	0.883249	87.2
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1975-2253 bp	0.883249	87.2