Hotspot

accD-psaI

LSC · 60461-61206 · 746 bp

Back to Solanum

Pi 0.0269

MarkerSeek score 66.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 241
pi: 0.0269
unique_haplotype_count: 128
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCGGGTACTTGGGATCCTAT	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	1049-1566 bp	0.994924	90.8
accD-psaI_p2	CCGGGTACTTGGGATCCTAT	CACCGACTACATTTCAGCCA	55.0	50.0	58.0	57.9	1304-1817 bp	0.969543	89.8
accD-psaI_p3	CACCCGTTTGATTGAACACG	CACCGACTACATTTCAGCCA	50.0	50.0	58.0	57.9	1074-1587 bp	0.964467	89.6
accD-psaI_p4	CACCCGTTTGATTGAACACG	ATGGATGAGATTGGGTCCCA	50.0	50.0	58.0	58.1	1116-1351 bp	0.548223	71.1
accD-psaI_p5	CCGGGTACTTGGGATCCTAT	ATGGATGAGATTGGGTCCCA	55.0	50.0	58.0	58.1	1346-1581 bp	0.548223	70.6