Hotspot

atpF-atpH

LSC · 13147-13547 · 401 bp

Back to Solanum

Pi 0.0189

MarkerSeek score 65.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 401
variable_sites: 125
pi: 0.0189
unique_haplotype_count: 86
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	580-615 bp	1.0	86.9
atpF-atpH_p2	GTGGCCCAAAGAAACGAAAG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	550-585 bp	1.0	86.8
atpF-atpH_p3	AGTGGCCCAAAGAAACGAAA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	58.2	57.5	551-586 bp	1.0	86.0
atpF-atpH_p4	TGGCCCAAAGAAACGAAAGA	CCTGAGGCAGAGGGAAAAAT	45.0	50.0	57.9	57.5	549-584 bp	0.954315	85.5
atpF-atpH_p5	TGGCCCAAAGAAACGAAAGA	GCAGAGGGAAAAATACGAGGT	45.0	47.6	57.9	58.0	543-578 bp	0.949239	81.4