Hotspot

trnK-UUU-rps16

LSC · 4413-5074 · 662 bp

Back to Solanum

Pi 0.0312
MarkerSeek score 70.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
662
variable_sites
266
pi
0.0312
unique_haplotype_count
146
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1268-1432 bp 0.994924 90.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1267-1431 bp 0.994924 90.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT CGTACGAGGAGAAAGCTTCC 50.0 55.0 58.2 58.1 857-1018 bp 0.979695 89.1
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TCTATCCCAATGAGCCGTCT 50.0 50.0 58.2 57.9 804-965 bp 0.964467 88.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCTATCCCAATGAGCCGTCT 50.0 50.0 58.2 57.9 803-964 bp 0.964467 88.5