Hotspot

clpP

LSC · 73118-75524 · 2407 bp

Back to Sobralia

Pi 0.0075

MarkerSeek score 58.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2407
variable_sites: 18
pi: 0.0075
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATAGAGCGGGTTGGCTTAGA	AAACCATTGTTACGTTTCCA	50.0	35.0	58.2	53.6	2565-2570 bp	1.0	43.9
clpP_p2	TAGAGCGGGTTGGCTTAGAT	AAACCATTGTTACGTTTCCA	50.0	35.0	58.2	53.6	2564-2569 bp	1.0	43.9
clpP_p3	CGGGTTGGCTTAGATCGATC	AAACCATTGTTACGTTTCCA	55.0	35.0	58.2	53.6	2559-2564 bp	1.0	43.9
clpP_p4	ATAGAGCGGGTTGGCTTAGA	AAAACCATTGTTACGTTTCCA	50.0	33.3	58.2	54.5	2566-2571 bp	1.0	43.0
clpP_p5	TAGAGCGGGTTGGCTTAGAT	AAAACCATTGTTACGTTTCCA	50.0	33.3	58.2	54.5	2565-2570 bp	1.0	43.0