Hotspot

psaC-ndhE

SSC · 123127-124012 · 886 bp

Back to Sobralia

Pi 0.00903

MarkerSeek score 63.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 886
variable_sites: 8
pi: 0.00903
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	AATACATCCGTGGGACAAGC	TTATAGCCATTGCAGCTGCT	50.0	45.0	58.0	57.6	1073-1074 bp	1.0	77.5
psaC-ndhE_p2	AATACATCCGTGGGACAAGC	TATAGCCATTGCAGCTGCTG	50.0	50.0	58.0	58.4	1072-1073 bp	1.0	77.1
psaC-ndhE_p3	AGCTCGAACACATTGGGTAC	TTATAGCCATTGCAGCTGCT	50.0	45.0	57.9	57.6	1056-1057 bp	1.0	77.0
psaC-ndhE_p4	AATACATCCGTGGGACAAGC	GTTATAGCCATTGCAGCTGC	50.0	50.0	58.0	57.6	1074-1075 bp	1.0	77.0
psaC-ndhE_p5	ACATCCGTCCCAAGGTATCA	TTATAGCCATTGCAGCTGCT	50.0	45.0	58.1	57.6	1098-1099 bp	1.0	76.7