Hotspot

rpl32-trnL-UAG

SSC · 118996-119796 · 801 bp

Back to Sobralia

Pi 0.0164
MarkerSeek score 62.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
801
variable_sites
13
pi
0.0164
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CTGTTTCTACCGGACAGTCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.9 57.9 897-905 bp 1.0 72.2
rpl32-trnL-UAG_p2 CTGTTTCTACCGGACAGTCA AGCAGCGTGTCTACCAATTT 50.0 45.0 56.9 57.8 890-898 bp 1.0 71.7
rpl32-trnL-UAG_p3 CTGTTTCTACCGGACAGTCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 56.9 57.8 896-904 bp 1.0 71.6
rpl32-trnL-UAG_p4 CTGTTTCTACCGGACAGTCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 56.9 57.8 895-903 bp 1.0 71.6
rpl32-trnL-UAG_p5 CTGTTTCTACCGGACAGTCA GAGCAGCGTGTCTACCAATT 50.0 50.0 56.9 58.3 891-899 bp 1.0 71.0