Hotspot

rps8-rpl14

LSC · 84990-85229 · 240 bp

Back to Sobralia

Pi 0.0346

MarkerSeek score 64.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 240
variable_sites: 8
pi: 0.0346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAATGTCCCTACCC	CGATTGCTCGGGAATTGAGA	55.0	50.0	58.1	58.1	321-330 bp	1.0	82.8
rps8-rpl14_p2	CGGCAATAATGTCCCTACCC	TTGCTCGGGAATTGAGACAG	55.0	50.0	58.1	57.9	318-327 bp	1.0	82.6
rps8-rpl14_p3	CGGCAATAATGTCCCTACCC	GCTCGGGAATTGAGACAGTT	55.0	50.0	58.1	57.9	316-325 bp	1.0	82.6
rps8-rpl14_p4	CGGCAATAATGTCCCTACCC	CGAATTTTTGGTGCGATTGC	55.0	45.0	58.1	57.2	334-343 bp	1.0	77.0
rps8-rpl14_p5	CGGCAATAATGTCCCTACCC	ATTGCTCGGGAATTGAGACA	55.0	45.0	58.1	57.2	319-328 bp	1.0	77.0