Hotspot

ycf4-cemA

LSC · 62965-63721 · 757 bp

Back to Sobralia

Pi 0.00793

MarkerSeek score 63.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 6
pi: 0.00793
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ACAAAAAGCTGCCGAATTGG	AAGATATGGGAGGGAAGCCA	45.0	50.0	57.8	57.8	851 bp	1.0	77.6
ycf4-cemA_p2	ACAAAAAGCTGCCGAATTGG	AGATATGGGAGGGAAGCCAA	45.0	50.0	57.8	57.8	850 bp	1.0	77.6
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	CCACCAGGGCAAAAAGAGTA	45.0	50.0	57.8	57.7	878 bp	1.0	77.0
ycf4-cemA_p4	TATTTCTTGCGCGTACCCAT	AAGATATGGGAGGGAAGCCA	45.0	50.0	57.7	57.8	829 bp	1.0	76.3
ycf4-cemA_p5	TATTTCTTGCGCGTACCCAT	AGATATGGGAGGGAAGCCAA	45.0	50.0	57.7	57.8	828 bp	1.0	76.3