Hotspot

trnF-GAA-ndhJ

LSC · 49536-50184 · 649 bp

Back to Sobralia

Pi 0.00617

MarkerSeek score 62.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 4
pi: 0.00617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	759 bp	1.0	79.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	749 bp	1.0	78.4
trnF-GAA-ndhJ_p3	CTGCTAGGATGATTCACGGG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.8	57.9	827 bp	1.0	78.1
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	802 bp	1.0	77.7
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	801 bp	1.0	77.7