Hotspot

petN-psbM

LSC · 29751-30496 · 746 bp

Back to Sobralia

Pi 0.0125

MarkerSeek score 67.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 9
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	CTTGCGCTTATTGCTACTGC	50.0	50.0	58.2	57.9	900-919 bp	1.0	81.0
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	839-858 bp	1.0	80.0
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	891-910 bp	1.0	79.8
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	892-911 bp	1.0	78.8
petN-psbM_p5	TAGTATGGGGAAGGAGTGGG	CTTGCGCTTATTGCTACTGC	55.0	50.0	57.5	57.9	843-862 bp	1.0	78.5