Hotspot

rpl22

LSC · 85227-85601 · 375 bp

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Pi 0.0096
MarkerSeek score 52.30
Primer available yes
Label rpl22

Feature Metadata

label_name
rpl22
feature_type
gene
region
LSC
length_bp
375
variable_sites
15
pi
0.0096
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22_p1 CGTGCAATTCCTTCGAAACC GATCCACTTGGCTACATCCG 50.0 55.0 58.0 58.1 1168-1370 bp 1.0 86.9
rpl22_p2 CGTGCAATTCCTTCGAAACC CAATCCACTGCCTTGATCCA 50.0 50.0 58.0 57.9 1182-1384 bp 1.0 86.8
rpl22_p3 CGTGCAATTCCTTCGAAACC AATCCACTGCCTTGATCCAC 50.0 50.0 58.0 57.9 1181-1383 bp 1.0 86.8
rpl22_p4 CGTGCAATTCCTTCGAAACC ACAATCCACTGCCTTGATCC 50.0 50.0 58.0 57.9 1183-1385 bp 1.0 86.8
rpl22_p5 AGGCCGGCAAGACAAATAAA GATCCACTTGGCTACATCCG 45.0 55.0 58.1 58.1 1678-1788 bp 0.7 71.9