Hotspot

psaC-ndhE

SSC · 120277-120785 · 509 bp

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Pi 0.0329
MarkerSeek score 88.50
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
509
variable_sites
50
pi
0.0329
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
88.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA TGTCATAGCTATTGCCGCTG 50.0 50.0 58.1 58.1 674-979 bp 1.0 87.4
psaC-ndhE_p2 CAGGCTCGTACACATTGAGT TGTCATAGCTATTGCCGCTG 50.0 50.0 57.6 58.1 634-939 bp 1.0 85.6
psaC-ndhE_p3 TTTCCAATACATCCGTGGGG TGTCATAGCTATTGCCGCTG 50.0 50.0 57.6 58.1 654-959 bp 1.0 85.0
psaC-ndhE_p4 ACATCCGTCCCAAGGTATCA TTGTCATAGCTATTGCCGCT 50.0 45.0 58.1 57.4 675-980 bp 1.0 83.5
psaC-ndhE_p5 AGCTTTACATCCGTCCCAAG TGTCATAGCTATTGCCGCTG 50.0 50.0 57.6 58.1 680-985 bp 0.9 80.7