Hotspot

trnP-UGG-psaJ

LSC · 68277-68765 · 489 bp

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Pi 0.0303
MarkerSeek score 81.30
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
489
variable_sites
43
pi
0.0303
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 627-652 bp 1.0 88.4
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 626-651 bp 1.0 88.4
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 638-663 bp 1.0 88.3
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 637-662 bp 1.0 88.3
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGCGTCTGGGAATAAACGAT 50.0 50.0 58.1 58.1 640-665 bp 1.0 88.3