Hotspot

ndhC-trnV-UAC

LSC · 50258-51998 · 1741 bp

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Pi 0.0303
MarkerSeek score 70.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1741
variable_sites
138
pi
0.0303
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.90
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ACCGTAGAGAAGTGGCCATA TACTCAGCCCATTCAATGCC 50.0 50.0 57.8 57.9 2526-2735 bp 1.0 87.4
ndhC-trnV-UAC_p2 TACCGTAGAGAAGTGGCCAT TACTCAGCCCATTCAATGCC 50.0 50.0 57.8 57.9 2527-2736 bp 1.0 87.4
ndhC-trnV-UAC_p3 TCTCTGGCCCTTCCCTAATC GGTCTGATTCAGGTGCGAAT 55.0 50.0 58.2 58.0 2165-2374 bp 0.9 83.5
ndhC-trnV-UAC_p4 ACCGTAGAGAAGTGGCCATA GGTCTGATTCAGGTGCGAAT 50.0 50.0 57.8 58.0 2586-2795 bp 0.9 83.3
ndhC-trnV-UAC_p5 TACCGTAGAGAAGTGGCCAT GGTCTGATTCAGGTGCGAAT 50.0 50.0 57.8 58.0 2587-2796 bp 0.9 83.3