Hotspot

psbM-trnD-GUC

LSC · 29178-30285 · 1108 bp

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Pi 0.0113
MarkerSeek score 72.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1108
variable_sites
43
pi
0.0113
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GCGGTAGGAACTAGAATGAACA GTACACTTCGCTGGGATTGT 45.5 50.0 57.9 57.9 1226-1276 bp 1.0 68.1
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC GTACACTTCGCTGGGATTGT 45.0 50.0 55.8 57.9 1214-1264 bp 1.0 67.3
psbM-trnD-GUC_p3 GCGGTAGGAACTAGAATGAACA AGTTCAATCGGTCAGAGCAC 45.5 50.0 57.9 57.9 1217-1256 bp 0.9 63.8
psbM-trnD-GUC_p4 AGAATGAACAGTGCAGTAGC AGTTCAATCGGTCAGAGCAC 45.0 50.0 55.8 57.9 1205-1244 bp 0.9 63.0
psbM-trnD-GUC_p5 AGCGGTAGGAACTAGAATGAAC AGTTCAATCGGTCAGAGCAC 45.5 50.0 57.6 57.9 1218-1257 bp 0.9 61.6