Hotspot

trnS-GCU-trnG-UCC

LSC · 8068-8979 · 912 bp

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Pi 0.0164
MarkerSeek score 74.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
912
variable_sites
57
pi
0.0164
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA TGAAAAACCGAAAGACCCCT 45.0 45.0 58.0 57.3 1029-1157 bp 1.0 82.0
trnS-GCU-trnG-UCC_p2 TGATGAGGGATAGCGGAGAG TGAAAAACCGAAAGACCCCT 55.0 45.0 58.1 57.3 1092-1220 bp 1.0 81.5
trnS-GCU-trnG-UCC_p3 GAGGGATAGCGGAGAGAGAG TGAAAAACCGAAAGACCCCT 60.0 45.0 58.2 57.3 1088-1216 bp 1.0 80.7
trnS-GCU-trnG-UCC_p4 AAGTGATGAGGGATAGCGGA TGAAAAACCGAAAGACCCCT 50.0 45.0 57.9 57.3 1095-1223 bp 0.9 76.6
trnS-GCU-trnG-UCC_p5 CAAGTGATGAGGGATAGCGG TGAAAAACCGAAAGACCCCT 55.0 45.0 57.8 57.3 1096-1224 bp 0.9 76.1