Hotspot

ycf1

SSC · 123860-129229 · 5370 bp

Back to Smelowskia

Pi 0.025

MarkerSeek score 55.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5370
variable_sites: 255
pi: 0.025
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAACGAATTCCAGGCTCCAT	TTTTGCATGGGATCTCTCGG	50.0	50.0	58.0	58.0	436-442 bp	1.0	86.8
ycf1_p2	TTGAACGAATTCCAGGCTCC	TTTTGCATGGGATCTCTCGG	50.0	50.0	58.2	58.0	438-444 bp	1.0	85.6
ycf1_p3	GTTTTACGCCTTTGGGAACG	TTTTGCATGGGATCTCTCGG	50.0	50.0	58.0	58.0	619-625 bp	1.0	85.4
ycf1_p4	TGCATTTCGCGTTTCCAATC	TTTTGCATGGGATCTCTCGG	45.0	50.0	58.0	58.0	1009-1015 bp	1.0	85.1
ycf1_p5	TGCATTTCGCGTTTCCAATC	CCCCCAACCTTTCCACTTTT	45.0	50.0	58.0	58.2	1273 bp	0.75	71.0