Hotspot

rpl32-trnL-CUA

SSC · 113741-114280 · 540 bp

Back to Smelowskia

Pi 0.0602

MarkerSeek score 81.90

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 540
variable_sites: 57
pi: 0.0602
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GCACTCTCGTACGGATTTCA	TAACCACTGGGACTGGAGAG	50.0	55.0	57.7	58.1	1423-1587 bp	1.0	87.5
rpl32-trnL-CUA_p2	ATGAGCACTCTCGTACGGAT	TAACCACTGGGACTGGAGAG	50.0	55.0	58.3	58.1	1520-1591 bp	0.75	74.3
rpl32-trnL-CUA_p3	GCACTCTCGTACGGATTTCA	TCGAACCGAGATGCTTTAGC	50.0	50.0	57.7	58.1	1038-1041 bp	0.5	61.7
rpl32-trnL-CUA_p4	AAATGAGCACTCTCGTACGG	TCGAACCGAGATGCTTTAGC	50.0	50.0	57.7	58.1	1044-1047 bp	0.5	61.7
rpl32-trnL-CUA_p5	AAATGAGCACTCTCGTACGG	TAACCACTGGGACTGGAGAG	50.0	55.0	57.7	58.1	1589-1593 bp	0.5	60.4