Hotspot

psaJ-rpl33

LSC · 66791-67232 · 442 bp

Back to Smelowskia

Pi 0.0434

MarkerSeek score 78.10

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 33
pi: 0.0434
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCAGATGCATTAACATTTCCCT	AACATCTTTACCCTTGGCCA	39.1	45.0	58.1	57.0	468-501 bp	1.0	56.4
psaJ-rpl33_p2	TCCAGATGCATTAACATTTCCC	AACATCTTTACCCTTGGCCA	40.9	45.0	56.7	57.0	468-501 bp	1.0	55.3
psaJ-rpl33_p3	CCAGATGCATTAACATTTCCCT	AACATCTTTACCCTTGGCCA	40.9	45.0	56.7	57.0	467-500 bp	1.0	55.2
psaJ-rpl33_p4	TCCAGATGCATTAACATTTCCCT	GAACATCTTTACCCTTGGCCA	39.1	47.6	58.1	58.2	469-502 bp	1.0	54.8
psaJ-rpl33_p5	TCCAGATGCATTAACATTTCCCT	CGAACATCTTTACCCTTGGC	39.1	50.0	58.1	56.8	470-503 bp	1.0	54.7