Hotspot

trnP-CCA-psaJ

LSC · 66293-66661 · 369 bp

Back to Smelowskia

Pi 0.0244

MarkerSeek score 61.10

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 369
variable_sites: 16
pi: 0.0244
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	458-463 bp	1.0	73.6
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	457-462 bp	1.0	73.6
trnP-CCA-psaJ_p3	ACAGGATTTGAACCCGTGAC	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	469-474 bp	1.0	73.4
trnP-CCA-psaJ_p4	CAGGATTTGAACCCGTGACA	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	468-473 bp	1.0	73.4
trnP-CCA-psaJ_p5	TGACAGGATTTGAACCCGTG	CGGTGCTACGGAAAGATATGT	50.0	47.6	58.1	57.9	471-476 bp	1.0	73.4