Hotspot

trnW-UGG-trnP-CCA

LSC · 66031-66218 · 188 bp

Back to Smelowskia

Pi 0.0567

MarkerSeek score 81.80

Primer available yes

Label trnW-UGG-trnP-CCA

Feature Metadata

label_name: trnW-UGG-trnP-CCA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 20
pi: 0.0567
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-UGG-trnP-CCA_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	256-268 bp	1.0	85.3
trnW-UGG-trnP-CCA_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	254-266 bp	1.0	85.3
trnW-UGG-trnP-CCA_p3	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	257-269 bp	1.0	85.2
trnW-UGG-trnP-CCA_p4	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	267-279 bp	1.0	85.1
trnW-UGG-trnP-CCA_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	268-280 bp	1.0	85.0