Hotspot

petA-psbJ

LSC · 62402-63381 · 980 bp

Back to Smelowskia

Pi 0.0398

MarkerSeek score 76.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 980
variable_sites: 73
pi: 0.0398
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGGCACAAATCTTTTTGGTTCT	CAGGATTAGGTTCATCCCTGT	36.4	47.6	57.2	56.8	1066-1081 bp	1.0	49.7
petA-psbJ_p2	TGGCACAAATCTTTTTGGTTCT	TCAGGATTAGGTTCATCCCTGT	36.4	45.5	57.2	58.3	1067-1082 bp	1.0	49.5
petA-psbJ_p3	TGGCACAAATCTTTTTGGTTCT	TCAGGATTAGGTTCATCCCTG	36.4	47.6	57.2	56.5	1067-1082 bp	1.0	49.5
petA-psbJ_p4	TGGCACAAATCTTTTTGGTTCTT	CAGGATTAGGTTCATCCCTGT	34.8	47.6	57.9	56.8	1066-1081 bp	1.0	49.5
petA-psbJ_p5	TGGCACAAATCTTTTTGGTTCTT	TCAGGATTAGGTTCATCCCTGT	34.8	45.5	57.9	58.3	1067-1082 bp	1.0	49.5