Hotspot

trnL-UUA-trnF-UUC

LSC · 47350-47861 · 512 bp

Back to Smelowskia

Pi 0.0612
MarkerSeek score 80.70
Primer available yes
Label trnL-UUA-trnF-UUC

Feature Metadata

label_name
trnL-UUA-trnF-UUC
feature_type
igs
region
LSC
length_bp
512
variable_sites
52
pi
0.0612
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UUA-trnF-UUC_p1 TGAATTCCAAGGTTTCGGCA TTTGAACTGGTGACACGAGG 45.0 50.0 58.0 58.1 1190-1369 bp 1.0 88.4
trnL-UUA-trnF-UUC_p2 TGAATTCCAAGGTTTCGGCA AAAGCCGAGCCAATAGGATG 45.0 50.0 58.0 58.0 1649-1831 bp 1.0 88.3
trnL-UUA-trnF-UUC_p3 TGAATTCCAAGGTTTCGGCA CCCGAAAGTTGGATAGGGTG 45.0 55.0 58.0 58.0 1889-2076 bp 1.0 88.0
trnL-UUA-trnF-UUC_p4 TGAATTCCAAGGTTTCGGCA CCGAAAGTTGGATAGGGTGG 45.0 55.0 58.0 58.0 1888-2075 bp 1.0 88.0
trnL-UUA-trnF-UUC_p5 CAAAAAGGGGATATGGCGGA AAAGCCGAGCCAATAGGATG 50.0 50.0 57.9 58.0 1584-1766 bp 1.0 87.9