Hotspot

trnT-ACA-trnL-UUA

LSC · 46081-46754 · 674 bp

Back to Smelowskia

Pi 0.039
MarkerSeek score 75.10
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
674
variable_sites
44
pi
0.039
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 TTTTCCGGCGATACGAACAT CTTCCATCGAGTCTCTGCAC 45.0 55.0 58.0 58.1 1555-1709 bp 1.0 86.6
trnT-ACA-trnL-UUA_p2 TTTTCCGGCGATACGAACAT GAGTCTCTGCACCTATCCCT 45.0 55.0 58.0 57.9 1547-1701 bp 1.0 86.6
trnT-ACA-trnL-UUA_p3 AAACTTGACCTGTTGACCCC CTTCCATCGAGTCTCTGCAC 50.0 55.0 57.9 58.1 1580-1734 bp 1.0 86.1
trnT-ACA-trnL-UUA_p4 AAACTTGACCTGTTGACCCC GAGTCTCTGCACCTATCCCT 50.0 55.0 57.9 57.9 1572-1726 bp 1.0 86.1
trnT-ACA-trnL-UUA_p5 TTTTCCGGCGATACGAACAT TTGGACCGGGAATTTCTTGG 45.0 50.0 58.0 58.1 2080-2208 bp 0.75 76.2