Hotspot

psbZ-trnG-GGC

LSC · 35856-36331 · 476 bp

Back to Smelowskia

Pi 0.0361

MarkerSeek score 80.60

Primer available yes

Label psbZ-trnG-GGC

Feature Metadata

label_name: psbZ-trnG-GGC
feature_type: igs
region: LSC
length_bp: 476
variable_sites: 32
pi: 0.0361
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GGC_p1	TCCTGATGGTTGGTCGAGTA	CGGATAGCGGGAATCGAAC	50.0	57.9	57.8	58.2	587-654 bp	1.0	76.7
psbZ-trnG-GGC_p2	TCCTGATGGTTGGTCGAGTA	GATAGCGGGAATCGAACCC	50.0	57.9	57.8	57.8	585-652 bp	1.0	76.5
psbZ-trnG-GGC_p3	TCCTGATGGTTGGTCGAGTA	GGATAGCGGGAATCGAACC	50.0	57.9	57.8	57.8	586-653 bp	1.0	76.5
psbZ-trnG-GGC_p4	CCGTTGTATTTGCGTCTCCT	CGGATAGCGGGAATCGAAC	50.0	57.9	58.3	58.2	603-670 bp	1.0	76.2
psbZ-trnG-GGC_p5	CCGTTGTATTTGCGTCTCCT	GATAGCGGGAATCGAACCC	50.0	57.9	58.3	57.8	601-668 bp	1.0	76.0