Hotspot

trnE-GAA-trnT-ACC

LSC · 30474-31289 · 816 bp

Back to Smelowskia

Pi 0.0329

MarkerSeek score 76.60

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 816
variable_sites: 48
pi: 0.0329
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	784-959 bp	1.0	86.9
trnE-GAA-trnT-ACC_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	763-938 bp	1.0	86.5
trnE-GAA-trnT-ACC_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	760-935 bp	1.0	86.4
trnE-GAA-trnT-ACC_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	746-921 bp	1.0	86.2
trnE-GAA-trnT-ACC_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	725-900 bp	1.0	86.2