Hotspot

psbM-trnD-GAC

LSC · 28703-29751 · 1049 bp

Back to Smelowskia

Pi 0.0361

MarkerSeek score 72.10

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 65
pi: 0.0361
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	TCGTAGTGTGGGGAAGAAGT	CGATCTTGCTAAGGATCCCG	50.0	55.0	58.0	57.9	2039-2083 bp	1.0	87.9
psbM-trnD-GAC_p2	CGTAGTGTGGGGAAGAAGTG	CGATCTTGCTAAGGATCCCG	55.0	55.0	57.9	57.9	2038-2082 bp	1.0	87.3
psbM-trnD-GAC_p3	TCGTAGTGTGGGGAAGAAGT	AAGTATGCCCCCATCGTCTA	50.0	50.0	58.0	57.9	2407-2451 bp	1.0	87.0
psbM-trnD-GAC_p4	CTCTCGTAGTGTGGGGAAGA	CGATCTTGCTAAGGATCCCG	55.0	55.0	58.2	57.9	2042-2086 bp	1.0	86.6
psbM-trnD-GAC_p5	CGTAGTGTGGGGAAGAAGTG	AAGTATGCCCCCATCGTCTA	55.0	50.0	57.9	57.9	2406-2450 bp	1.0	86.4