Hotspot

rpoB-trnC-UGC

LSC · 26133-27245 · 1113 bp

Back to Smelowskia

Pi 0.0308

MarkerSeek score 67.80

Primer available yes

Label rpoB-trnC-UGC

Feature Metadata

label_name: rpoB-trnC-UGC
feature_type: igs
region: LSC
length_bp: 1113
variable_sites: 65
pi: 0.0308
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-UGC_p1	CACGAGCCCATATTCTTGCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1680-1689 bp	1.0	83.7
rpoB-trnC-UGC_p2	ATTGGATCCCCACCTACACA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1879-1888 bp	1.0	83.4
rpoB-trnC-UGC_p3	ATTGGATCCCCACCTACACA	ACCACAAGCAAATGGACACT	50.0	45.0	58.0	57.9	2352 bp	0.5	59.8
rpoB-trnC-UGC_p4	CACGAGCCCATATTCTTGCT	ACCACAAGCAAATGGACACT	50.0	45.0	58.0	57.9	2153 bp	0.5	59.7
rpoB-trnC-UGC_p5	GTTCCCTCTTTTCCATCCCC	ACCACAAGCAAATGGACACT	55.0	45.0	57.9	57.9	1665 bp	0.5	59.0