Hotspot

trnS-AGC-trnG-GGA

LSC · 7796-8406 · 611 bp

Back to Smelowskia

Pi 0.0512
MarkerSeek score 87.30
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
611
variable_sites
56
pi
0.0512
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
87.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTT 50.0 45.0 57.8 56.5 670-761 bp 1.0 76.6
trnS-AGC-trnG-GGA_p2 CCAACGCTTTAGTCCACTCA ATCAAACCGAGAGACCCTTT 50.0 45.0 57.8 56.5 669-760 bp 1.0 76.6
trnS-AGC-trnG-GGA_p3 AACGCTTTAGTCCACTCAGC AATCAAACCGAGAGACCCTT 50.0 45.0 58.2 56.5 668-759 bp 1.0 76.3
trnS-AGC-trnG-GGA_p4 AACGCTTTAGTCCACTCAGC ATCAAACCGAGAGACCCTTT 50.0 45.0 58.2 56.5 667-758 bp 1.0 76.3
trnS-AGC-trnG-GGA_p5 CCAACGCTTTAGTCCACTCA AATCAAACCGAGAGACCCTTT 50.0 42.9 57.8 57.2 670-761 bp 1.0 74.7