Hotspot

trnH-CAC-psbA

LSC · 77-297 · 221 bp

Back to Smelowskia

Pi 0.0538

MarkerSeek score 79.00

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 22
pi: 0.0538
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	294-373 bp	1.0	87.2
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	297-376 bp	1.0	87.0
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	TGTTAGGTGTAGCCGGTGTA	50.0	50.0	57.9	58.1	716-795 bp	1.0	82.5
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	279-358 bp	0.75	73.8
trnH-CAC-psbA_p5	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	280-359 bp	0.75	73.6