Hotspot

matK

LSC · 2258-3772 · 1515 bp

Back to Sloanea

Pi 0.00145
MarkerSeek score 52.30
Primer available yes
Label matK

Feature Metadata

label_name
matK
feature_type
gene
region
LSC
length_bp
1515
variable_sites
5
pi
0.00145
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK_p1 GGGATAATTTCTATTTAGAAGGTCTCA TCCCCTACCTTTGGTTCAAA 33.3 45.0 56.7 56.2 1580 bp 1.0 40.3
matK_p2 AGGGATAATTTCTATTTAGAAGGTCTC TCCCCTACCTTTGGTTCAAA 33.3 45.0 56.5 56.2 1581 bp 1.0 40.3
matK_p3 GGGATAATTTCTATTTAGAAGGTCTCA AATCCCCTACCTTTGGTTCA 33.3 45.0 56.7 56.0 1582 bp 1.0 40.3
matK_p4 GGGATAATTTCTATTTAGAAGGTCTCA ATCCCCTACCTTTGGTTCAA 33.3 45.0 56.7 56.0 1581 bp 1.0 40.3
matK_p5 AGGGATAATTTCTATTTAGAAGGTCT TCCCCTACCTTTGGTTCAAA 30.8 45.0 55.5 56.2 1581 bp 1.0 40.3