Hotspot

ndhF-trnL-UAG

SSC · 116854-117966 · 1113 bp

Back to Sloanea

Pi 0.00665
MarkerSeek score 53.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1113
variable_sites
13
pi
0.00665
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 GGGAAGTGTAACAAAAGGTATGA AGCGTGTCTACCGATTTCAC 39.1 50.0 56.5 58.0 1190-1197 bp 1.0 45.2
ndhF-trnL-UAG_p2 GGGAAGTGTAACAAAAGGTATGA CAGCGTGTCTACCGATTTCA 39.1 50.0 56.5 58.0 1191-1198 bp 1.0 45.1
ndhF-trnL-UAG_p3 GGGAAGTGTAACAAAAGGTATGA TCCTAAGAGCAGCGTGTCTA 39.1 50.0 56.5 57.9 1200-1207 bp 1.0 44.2
ndhF-trnL-UAG_p4 GGGAAGTGTAACAAAAGGTATGA CCTAAGAGCAGCGTGTCTAC 39.1 55.0 56.5 57.8 1199-1206 bp 1.0 43.6
ndhF-trnL-UAG_p5 GGGAAGTGTAACAAAAGGTATGA CTAAGAGCAGCGTGTCTACC 39.1 55.0 56.5 57.8 1198-1205 bp 1.0 43.6