Hotspot

trnN-GUU-ndhF

IRb · 113088-114615 · 1528 bp

Back to Sloanea

Pi 0.00609

MarkerSeek score 45.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1528
variable_sites: 23
pi: 0.00609
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GGCAGAATAGCAGAGCAAGT	GGGGTTGGTGTTACGAGTTT	50.0	50.0	58.0	58.0	2052-2130 bp	1.0	82.8
trnN-GUU-ndhF_p2	CATCATGATCGGGATAGCGG	GGGGTTGGTGTTACGAGTTT	55.0	50.0	58.1	58.0	2218-2296 bp	1.0	82.3
trnN-GUU-ndhF_p3	GACTTACCTACACCGGGGTA	GGGGTTGGTGTTACGAGTTT	55.0	50.0	57.9	58.0	1848-1926 bp	1.0	82.2
trnN-GUU-ndhF_p4	GGCAGAATAGCAGAGCAAGT	AAGAAACATGACGCGGTCTT	50.0	45.0	58.0	58.1	2599-2677 bp	1.0	81.4
trnN-GUU-ndhF_p5	GGCAGAATAGCAGAGCAAGT	AGAAACATGACGCGGTCTTT	50.0	45.0	58.0	58.1	2598-2676 bp	1.0	81.4