Hotspot

rps8-rpl14

LSC · 84633-84841 · 209 bp

Back to Sloanea

Pi 0.0153

MarkerSeek score 59.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 209
variable_sites: 5
pi: 0.0153
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GTCAGCATTTCGTATCGAGGT	ACGAATTTTTGGTGCGATCG	47.6	45.0	58.2	57.8	324-339 bp	1.0	73.6
rps8-rpl14_p2	TGTCAGCATTTCGTATCGAGG	ACGAATTTTTGGTGCGATCG	47.6	45.0	58.2	57.8	325-340 bp	1.0	73.6
rps8-rpl14_p3	TCAGCATTTCGTATCGAGGT	ACGAATTTTTGGTGCGATCG	45.0	45.0	56.8	57.8	323-338 bp	1.0	73.1
rps8-rpl14_p4	CCATGTCAGCATTTCGTATCG	ACGAATTTTTGGTGCGATCG	47.6	45.0	57.5	57.8	328-343 bp	1.0	71.0
rps8-rpl14_p5	TCAGCATTTCGTATCGAGGTT	ACGAATTTTTGGTGCGATCG	42.9	45.0	57.5	57.8	323-338 bp	1.0	70.9