Hotspot

ndhK-ndhC

LSC · 53440-53512 · 73 bp

Back to Sloanea

Pi 0.0167
MarkerSeek score 60.30
Primer available yes
Label ndhK-ndhC

Feature Metadata

label_name
ndhK-ndhC
feature_type
igs
region
LSC
length_bp
73
variable_sites
2
pi
0.0167
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhK-ndhC_p1 AGAGAAGCGGCCATAAACTG TGGTTCAGTCTATGCATGGC 50.0 50.0 58.0 58.0 231-234 bp 1.0 81.1
ndhK-ndhC_p2 AGAGAAGCGGCCATAAACTG GTTCAGTCTATGCATGGCGA 50.0 50.0 58.0 58.1 229-232 bp 1.0 81.0
ndhK-ndhC_p3 AGAGAAGCGGCCATAAACTG CATGGCGAAAAGGAGCATTG 50.0 50.0 58.0 58.1 217-220 bp 1.0 80.9
ndhK-ndhC_p4 AGAGAAGCGGCCATAAACTG TGGGCAATGAGTTTCGATGT 50.0 45.0 58.0 57.8 305-308 bp 1.0 79.5
ndhK-ndhC_p5 ACCATAGAGAAGCGGCCATA TGGTTCAGTCTATGCATGGC 50.0 50.0 58.3 58.0 236-239 bp 1.0 79.1