Hotspot

rpoB-trnC-GCA

LSC · 28253-29468 · 1216 bp

Back to Sloanea

Pi 0.00526

MarkerSeek score 47.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 11
pi: 0.00526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCATTTCCATCCCCCAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.1	58.1	1298-1302 bp	1.0	71.5
rpoB-trnC-GCA_p2	TTCCCTCATTTCCATCCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.1	58.1	1303-1307 bp	1.0	71.5
rpoB-trnC-GCA_p3	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1304-1308 bp	1.0	71.4
rpoB-trnC-GCA_p4	CCCGGGTATTGTAGACATTCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	1320-1324 bp	1.0	71.0
rpoB-trnC-GCA_p5	CCGGGTATTGTAGACATTCCC	CCGGATTTGAACTGGGGAAA	52.4	50.0	57.9	58.1	1319-1323 bp	1.0	71.0