Hotspot

ycf45

Genome · 114528-115892 · 1365 bp

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Pi 0.0812

MarkerSeek score 79.50

Primer available yes

Label ycf45

Feature Metadata

label_name: ycf45
feature_type: gene
region: Genome
length_bp: 1365
variable_sites: 218
pi: 0.0812
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	TCCACTGCTCTACCAACTGA	GGAAATGTTGCCCGTAAACG	50.0	50.0	58.0	58.0	2154-2245 bp	1.0	91.3
ycf45_p2	TCCACTGCTCTACCAACTGA	GCCAAGGGTTATAGAGGTGC	50.0	55.0	58.0	58.0	2112-2203 bp	1.0	91.3
ycf45_p3	TCCACTGCTCTACCAACTGA	CCTACAACTTGCCAAGGGTT	50.0	50.0	58.0	58.0	2213 bp	0.2	47.9
ycf45_p4	AATCGCAAAGCACCGAAATG	CCTACAACTTGCCAAGGGTT	45.0	50.0	58.0	58.0	2354 bp	0.2	47.8
ycf45_p5	TTTGCGTGAAAGTGGATCCA	CCTACAACTTGCCAAGGGTT	45.0	50.0	58.0	58.0	2471 bp	0.2	47.8