Hotspot

tufA

Genome · 48426-49655 · 1230 bp

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Pi 0.0565

MarkerSeek score 80.30

Primer available yes

Label tufA

Feature Metadata

label_name: tufA
feature_type: gene
region: Genome
length_bp: 1230
variable_sites: 141
pi: 0.0565
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tufA_p1	AGGGGTGGTCGAGTAAAAGA	GGTATGGCCAAGTGGTAAGG	50.0	55.0	58.0	57.9	2647-2671 bp	0.6	69.6
tufA_p2	AGGGGTGGTCGAGTAAAAGA	GCCTAGTGATAGTGCGTGAG	50.0	55.0	58.0	57.9	2466-2490 bp	0.6	69.1
tufA_p3	AGGGGTGGTCGAGTAAAAGA	TAGTGATAGTGCGTGAGGGT	50.0	50.0	58.0	57.9	2463-2487 bp	0.6	69.0
tufA_p4	AGGGGTGGTCGAGTAAAAGA	TGGTAGACACGACGGACTTA	50.0	50.0	58.0	57.8	2495-2519 bp	0.6	68.6
tufA_p5	TATTCACGACAACGCGTAGG	GGTATGGCCAAGTGGTAAGG	50.0	55.0	58.1	57.9	2142 bp	0.2	46.8