Hotspot

thiG

Genome · 53409-54218 · 810 bp

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Pi 0.0881

MarkerSeek score 78.70

Primer available yes

Label thiG

Feature Metadata

label_name: thiG
feature_type: gene
region: Genome
length_bp: 810
variable_sites: 138
pi: 0.0881
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
thiG_p1	AATGCTGCACGATTCTTCCT	TGGTCGTAGGTTCAAGTCCT	45.0	50.0	57.9	58.0	1299-1335 bp	0.6	73.6
thiG_p2	ATGCTGCACGATTCTTCCTT	TGGTCGTAGGTTCAAGTCCT	45.0	50.0	57.9	58.0	1298-1334 bp	0.6	73.6
thiG_p3	AATGCTGCACGATTCTTCCT	TTCAAGTCCTACCTGGGGAG	45.0	55.0	57.9	58.0	1289-1325 bp	0.6	73.3
thiG_p4	ATGCTGCACGATTCTTCCTT	TTCAAGTCCTACCTGGGGAG	45.0	55.0	57.9	58.0	1288-1324 bp	0.6	73.3
thiG_p5	ATGCTGCACGATTCTTCCTT	CTCAGTGGTAGAGCAATCGG	45.0	55.0	57.9	57.8	1330-1366 bp	0.6	71.9