Hotspot

petN-trnD(guc)

Genome · 87798-87986 · 189 bp

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Pi 0.0612

MarkerSeek score 83.90

Primer available yes

Label petN-trnD(guc)

Feature Metadata

label_name: petN-trnD(guc)
feature_type: igs
region: Genome
length_bp: 189
variable_sites: 22
pi: 0.0612
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnD(guc)_p1	ACATAGTCATTACGCCAGCC	AAGTTGCGAGTTCGAGTCTC	50.0	50.0	57.8	57.9	252-262 bp	1.0	88.4
petN-trnD(guc)_p2	ACATAGTCATTACGCCAGCC	GAAGTTGCGAGTTCGAGTCT	50.0	50.0	57.8	57.9	253-263 bp	1.0	88.4
petN-trnD(guc)_p3	CGTTTCGACCCCAAACTACT	AAGTTGCGAGTTCGAGTCTC	50.0	50.0	57.8	57.9	291-301 bp	1.0	87.7
petN-trnD(guc)_p4	CGTTTCGACCCCAAACTACT	GAAGTTGCGAGTTCGAGTCT	50.0	50.0	57.8	57.9	292-302 bp	1.0	87.7
petN-trnD(guc)_p5	TAAAAACCGTTTCGACCCCA	AAGTTGCGAGTTCGAGTCTC	45.0	50.0	57.7	57.9	298-308 bp	1.0	86.0