Hotspot

trnG(gcc)-psbZ

Genome · 82936-83083 · 148 bp

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Pi 0.116
MarkerSeek score 79.90
Primer available yes
Label trnG(gcc)-psbZ

Feature Metadata

label_name
trnG(gcc)-psbZ
feature_type
igs
region
Genome
length_bp
148
variable_sites
33
pi
0.116
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG(gcc)-psbZ_p1 TCTACCACTGAGCTATGCCC TGTTGTTGCAATTGCTACTGC 55.0 42.9 58.6 58.3 213-216 bp 1.0 75.1
trnG(gcc)-psbZ_p2 CTCTACCACTGAGCTATGCC TGTTGTTGCAATTGCTACTGC 55.0 42.9 57.2 58.3 214-217 bp 1.0 73.3
trnG(gcc)-psbZ_p3 TCTACCACTGAGCTATGCCC GTTGTTGCAATTGCTACTGC 55.0 45.0 58.6 56.5 212-215 bp 1.0 73.2
trnG(gcc)-psbZ_p4 CTCTACCACTGAGCTATGCC GTTGTTGCAATTGCTACTGC 55.0 45.0 57.2 56.5 213-216 bp 1.0 71.4
trnG(gcc)-psbZ_p5 GTTGCGCTCTACCACTGAG TGTTGTTGCAATTGCTACTGC 57.9 42.9 58.3 58.3 220-223 bp 1.0 69.7