Hotspot

rns-ycf89

Genome · 71303-71576 · 274 bp

Back to Skeletonema

Pi 0.167

MarkerSeek score 78.60

Primer available yes

Label rns-ycf89

Feature Metadata

label_name: rns-ycf89
feature_type: igs
region: Genome
length_bp: 274
variable_sites: 89
pi: 0.167
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-ycf89_p1	TCTCGTACGACTTGCATGTG	TTGGTATTCAGAACGCCCAG	50.0	50.0	58.0	57.9	517-527 bp	0.6	71.0
rns-ycf89_p2	CATTGCGGAAAATTCCCCAC	TTGGTATTCAGAACGCCCAG	50.0	50.0	58.0	57.9	799-809 bp	0.6	70.5
rns-ycf89_p3	TATTGCTCCGTCAGGCTTTC	TTGGTATTCAGAACGCCCAG	50.0	50.0	58.0	57.9	822-832 bp	0.6	70.2
rns-ycf89_p4	CAACCCATAGGCAGTCTTCC	TTGGTATTCAGAACGCCCAG	55.0	50.0	58.0	57.9	851-861 bp	0.6	69.8
rns-ycf89_p5	AGCAGTCGTTTCCAACTGTT	TTGGTATTCAGAACGCCCAG	45.0	50.0	58.0	57.9	599 bp	0.2	46.8