Hotspot

dnaK-rpl3

Genome · 33906-34258 · 353 bp

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Pi 0.143

MarkerSeek score 79.50

Primer available yes

Label dnaK-rpl3

Feature Metadata

label_name: dnaK-rpl3
feature_type: igs
region: Genome
length_bp: 353
variable_sites: 92
pi: 0.143
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK-rpl3_p1	TCCAGCATCAACAGTTGCTT	GTTACCAAGTTGACCTGCCA	45.0	50.0	58.0	58.0	1295-1309 bp	0.6	72.8
dnaK-rpl3_p2	TCCAGCATCAACAGTTGCTT	TGTTACCAAGTTGACCTGCC	45.0	50.0	58.0	58.0	1296-1310 bp	0.6	72.8
dnaK-rpl3_p3	CCAGCATCAACAGTTGCTTG	GTTACCAAGTTGACCTGCCA	50.0	50.0	57.9	58.0	1294-1308 bp	0.6	72.4
dnaK-rpl3_p4	TCCAGCATCAACAGTTGCTT	CAGGTTTTCCAGGAACAGCT	45.0	50.0	58.0	58.0	1386 bp	0.2	47.8
dnaK-rpl3_p5	CCAGCATCAACAGTTGCTTG	CAGGTTTTCCAGGAACAGCT	50.0	50.0	57.9	58.0	1385 bp	0.2	47.4