Hotspot

ycf89-rns

Genome · 11242-11515 · 274 bp

Back to Skeletonema

Pi 0.168

MarkerSeek score 78.70

Primer available yes

Label ycf89-rns

Feature Metadata

label_name: ycf89-rns
feature_type: igs
region: Genome
length_bp: 274
variable_sites: 90
pi: 0.168
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf89-rns_p1	TTGGTATTCAGAACGCCCAG	TCTCGTACGACTTGCATGTG	50.0	50.0	57.9	58.0	517-527 bp	0.6	71.0
ycf89-rns_p2	TTGGTATTCAGAACGCCCAG	CATTGCGGAAAATTCCCCAC	50.0	50.0	57.9	58.0	799-809 bp	0.6	70.5
ycf89-rns_p3	TTGGTATTCAGAACGCCCAG	TATTGCTCCGTCAGGCTTTC	50.0	50.0	57.9	58.0	822-832 bp	0.6	70.2
ycf89-rns_p4	TTGGTATTCAGAACGCCCAG	CAACCCATAGGCAGTCTTCC	50.0	55.0	57.9	58.0	851-861 bp	0.6	69.8
ycf89-rns_p5	TTGGTATTCAGAACGCCCAG	AGCAGTCGTTTCCAACTGTT	50.0	45.0	57.9	58.0	599 bp	0.2	46.8