Hotspot

ycf1

SSC · 124372-129783 · 5412 bp

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Pi 0.00923
MarkerSeek score 45.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5412
variable_sites
116
pi
0.00923
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGAACTCCGAGGTGTCGTAT TCCGGCCAAACTAACCAAAA 50.0 45.0 57.9 57.9 720-747 bp 1.0 82.0
ycf1_p2 AGAACTCCGAGGTGTCGTAT ACGCAAACGAACCTTACCTT 50.0 45.0 57.9 58.0 936-963 bp 0.833333 75.2
ycf1_p3 AGAACTCCGAGGTGTCGTAT AAAGATGTACGAATCCGGCC 50.0 50.0 57.9 58.1 733 bp 0.166667 45.3
ycf1_p4 AGAACTCCGAGGTGTCGTAT CCTTTGGTTCTGTTCACCCA 50.0 50.0 57.9 57.9 920 bp 0.166667 45.1
ycf1_p5 AGAACTCCGAGGTGTCGTAT ACCTTTGGTTCTGTTCACCC 50.0 50.0 57.9 57.9 921 bp 0.166667 45.1